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KMID : 0357319940290050489
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Journal of the Korean Society for Microbiology
1994 Volume.29 No. 5 p.489 ~ p.498
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Biotyping, Plasmid and Chromosomal DNA Analysis in Multiresistant Acinetobater baumannii Isolates
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Abstract
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During the last few decades, Acinetobacter baumannii strains have gained increasing nosocomial significance. The recently increasing frequency of multiresistant A. baumannii isolates at Chonnam University Hospital led us to investigate the
epidemic
strains. The purpose of this study was to detemine whether plasmid DNA analysis, biotyping and chromosomal DNA analysis of A. baumannii were feasible and to establish their relationships with respect to defining nosocomial cross-infections.
A total of 43 multiresistant Acinetobacter baumannii strains isolated from 43 hospitalized patients between January and September 1993 were analyzed. The plasmid DNA of isolates was obtained by a modification of alkaline lysis method, and
biotyping
was
done by a commercially available carbon assimilation system, ATB 32GN System (API System, La Balme-Les Grottes, France). Chromosomal DNA analysis was perfomed in strains which showed the same plasmid DNA patterns, with the pulsed field gel
electrophoresis (PFGE) System.
Foty(93%) A. baumannii strains of the 43 isolates were found to contain plasmid DNA. Plasmid DNA patterns were distinct in 29 isolates (they differed from each other and all others), but identical in two groups of isolates(seven and four
isolates,
respectively). GroupⅠisolates consisted of seven blood isolates from seven neonatal patients of NICU, and GroupⅡconsisted of four sputum isolates from four patients of ICU. Biotyping by ATB 32GN system was not successful in identifying sameness
among
group isolates nor differences among other isolates. Each group isolates showed identical ApaI and SmaI digestion patterns of chromosomal DNA by using PFGE.
These results suggest that plasmid DNA analysis and PFGE of chromosomal DNA digested by ApaI and SmaI restriction enzymes are very effective methods for the epidemiologic investigation of infection due to multiresistant A. baumannii strains, but
biotypying alone, however, appears to be insufficient.
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KEYWORD
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